The promoter fragments of IGFBP3 160 1414, 160, 5 AAACTCGAGGCATTCGTGTGTACCTCGTG 3; 1414, 5 CCCGAGCTCTGATCTTCCCCTGTCCACTC 3, HIF 1a 80 1871, 80, 5 AAACTCGAGCTCTCCTCAGGTGGCTTGTC 3; 1871, 5 CCCGAGCTCGAGTTGCAGTGAGCCGAAAT 3, and HIF 2a 46 1222, 46, 5 AAACTCGAGGAGGACAAGCTGGCAGAGAC 3; 1222, 5 CCCGAGCTCGTGTTCCGCATTTTGGAAGT 3 were generated by chromosomal DNA extraction from P0, amplified by Q5 High Fidelity DNA Polymerase NEB, Ipswich, MA, USA, and constructed into pGL3 Promega, Madison, WI, USA
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