what is lasix The marker genes representing undifferentiated spermatogonia Nanos3, differentiated spermatogonia c Kit, undifferentiated and differentiated spermatogonia Sohlh1, differentiated spermatogonia and preleptotene spermatocytes Stra8, spermatids Prm2, Leydig cells Hsd3b1, and Sertoli cells Sox9 were analyzed by qRT PCR