2, when turning on a gene such as DTA, it requires deletion of an upstream floxed STOP cassette to allow transcription of the gene; typically, a single copy heterozygous of the gene is all that is necessary to kill a cell, as is the case with DTA, or label a cell using a reporter gene such as green fluorescent protein GFP.
doxycycline coronavirus An amendment to this paper has been published and can be accessed via the original article.